EnviroGard�, Modern Water

7540000 EnviroGard Microcystins Plate Kit by Modern Water

Envirogard® is a rapid laboratory enzyme immunoassay method for the analysis of water. It uses a plate technology to detect the analyte of concern.

Features:

• Rapid lab testing method for the detection of microcystins and nodularins in drinking and environmental water samples
• Conforms to ISO standards for environmental immunoassays such as ISO 15089
• Quantiative data results with excellent analytical precision
• Test up to 88 samples* at once for increased cost efficiency
• Results available in approximately 120 minutes
• Microtiter plate immunoassay
• Ideal for source water monitoring

Test result type

• Quantitative data

Samples per kit

• 96 well test kit (tests up to 88 samples*)

Assay range

• 0.1 ppb to 1.6 ppb
• Extended range available

Sampling time

• Sample-to-data in approximately 120 minutes

Specificity

The proprietary antibody used in this assay detects microcystins and nodularins. The crossreactivity of the Microcycstins EnviroGard® Plate Kit for various microcystin variants is shown below at 50% B/B₀

Basic Test Procedure

• Prepare standards
• Add 100 uL of sample or standard
• Incubate for 30 minutes
• Add 100 uL enzyme conjugate
• Incubate for 30 minutes
• Decant and wash five times
• Add 100 uL colour solution
• Incubate for 30 minutes
• Stop the reaction and read colour at 450nm

Test kit components

• Antibody coated microtiter plate strips, enzyme conjugate, colour development and stop reagent for analysis of 96 wells
• One vial each of 0.1, 0.2, 0.4, 0.56, 0.8 and 1.6 ppb microcystin-LR surrogate (nontoxic) calibrators
• Test kit instructions
• Optional 1.5 ppb check standard

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use for up to eight hours
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• Microcystins EnviroGard® plate (7540000)

Required test equipment

• 10 cc syringes
• 0.45 micron non-sterile filter units
• Pipette to deliver 100 mL
• Distilled or DI water for rinsing wells
• Microtiter plate reder or stip reader
• Timer
• Parafilm or tape
• Marker pen

Other recommended materials

• Orbital shaker
• Multi-channel pipette
• Repeater pipette
• Calculator
• Microtiter plate washer
• Solid extraction devices

7310000 EnviroGard DDT Soil Kit by Modern Water

Envirogard® is a rapid field enzyme immunoassay method for the analysis of soil remediation, assessment and industrial testing. It uses a coated tube technology to detect the analyte of concern.

Features:

• Rapid and simple field testing procedures
• Semi-quantitative data results at set calibrator (action) levels
• Test up to 17 samples at once for increased cost efficiency
• Results available in less than 30 minutes
• Coated tube immunoassay
• EPA SW-846 Method #4042

Test result type

• Semi-quantitative or qualitative data

Samples per kit

• Up to 17 soil sample test results can be obtained per kit, depending upon the number of runs and action levels used.
• Typical applications use two or three calibrators and one negative control.

Assay range

• Semi-quantitative results (greater or less than) at calibrator (action) levels of 0.2, 1.0 and 10.0 ppm DDT
• Minimum detection level (MDL) is 0.2 ppm DDT.
• No maximum screening level as extracts can be diluted into test range

Sample preparation

• Soil samples require prior extraction using the MWI Sample Extraction Kit (sold separately).
• The Sample Extraction Kit provides material for 12 soil sample extractions with methanol.

Sampling time

• Soil extraction time is typically 2 minutes per sample plus assay run time of approximately 30 minutes.

Specificity

The EnviroGard DDT immunoassay test does not differentiate between DDT and other organochlorines. The table below shows compounds at the minimum detection limit (MDL) -an approximate concentration required to yield a positive result atthe lowest standard. The IC50 is the concentration required to inhibit one half of the color produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Basic Test Procedure

• Extract the soil sample using the MWI Sample Extraction Kit
• Pipet DDT assay diluent into the antibody coated tubes, then add either DDT calibrator or sample extract to the tubes along with DDT enzyme conjugate.
• Wait 15 minutes, then decant and wash the tubes.
• Add substrate.
• Wait for 10 minutes, add stop solution
• Read the absorbance using a photometer, and interpret the results. (Refer to DDT Soil Test Kit Instruction Guide procedures.)

Test kit components

• 20 antibody coated tubes
• Enzyme conjugate, colour developing and colour stop solution reagents
• Calibrators for 0.2, 1.0 and 10.0 ppm DDT.
• Negative control calibrator
• Test tube rack and pipet tips.
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Components must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight
• Portable spectrophotometer battery should be charged prior to use

Required test materials

• EnviroGard® DDT Soil Kit (7310000)
• Sample extraction kit (soil)(7310000EB)

Required test equipment

• Field soil lab (Accessory kit) (6050400) which includes: Repeating pipette*, Adjustable pipette*, Balance*, Repeating pipette tips*, Timer*
• Differential spectrophotometer - 110V (6000001), 220V (6000002)
• Field lab system rental (includes items marked with * above) (6997020)

Other recommended materials

• Latex gloves
• Absorbent paper for blotting test tubes
• Liquid and solid waste containers
• Calculator

7020301 PCB EnviroGard 12T Soil Test Kit by Modern Water

Envirogard® is a rapid field enzyme immunoassay method for the analysis of soil remediation, assessment and industrial testing. It uses a coated tube technology to detect the analyte of concern.

Features:

• Convenient and rapid testing in the field or labatory
• Tests up to 10 samples at once
• Two detection levels of your choice are provided with each sample
• High level of accuracy
• EPA SW-846 Method #4020

Test result type

• Semi-quantitative or qualitative data
• Knowledge of specific aroclor(s) present will aid accuracy

Samples per kit

• Dependent on batch size and number of detection levels
• Each sample can be tested one at a time or in batches of two to ten samples

Assay range

• Soil: 0.5 ppm to 500 ppm with dilutions
• Wipes: 5.0 ug/100cm² to 5000 ug/100cm² with dilutions

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately)
• The sample extraction kit provides materials for 12 soil sample extractions with methanol

Sampling time

• Approximately two minutes per sample plus assay run time of approximately 30 minutes

Basic Test Procedure

• Transfer soil or wipe to extraction jar
• Cap and shake for one minute
• Filter extracted sample
• Pipette and dilute samples with dilution ampule
• Transfer 1 ml of buffer to glass buffer tubes
• Pipette and transfer sample to buffer tubes
• Fit antibody coated tubes on buffer tubes; invert and mix;return to antibody coated tube
• Incubate for 10 minutes
• Connect antibody coated and conjugate tubes; invert and mix, return to antibody coated tube
• Incubate five minutes
• Wash antibody coated tubes vigorously four times
• Using a repeater pipette at setting two and 5 mL tip,transfer 200 uL substrateA to each tube
• Add 200 uL substrate B to each tube and mix
• Inclubate for 2.5 minutes
• Add 200 uL STOP to each tube
• Read tubes in photometer.Record data

Test kit components

• 48 antibody coated tubes and PCB enzyme conjugate vials
• 48 glass buffer tubes
• Substrate and stop solution reagents
• PCB standard
• Pipette tips and bulb pipettes
• Buffer
• Amber vials
• Wash solution
• 12 ampule crackers

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging.
• Store at ambient temperature 64° to 81°F (18° to 27°C)
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not freeze test kit components

Required test materials

• PCB EnviroGard 12T accessory kit (6050400)
• PCB EnviroGard 12T accessory kit rental (Includes all equipment required to operate test and interpret results)(6997020)

Required test equipment

• PCB EnviroGard 12T soil test kit (7020301)
• PCB soil sample extraction kit (7020301EB)
• EnviroGard PCB wipe sample extraction kit (7020301WA)

Other recommended materials

• Absorbent paper
• Liquid waste container
• Disposable gloves
• Marker pen
• Liquid and solid waste containers

7210000 EnviroGard Triazine Test Kit by Modern Water

Envirogard® is a rapid field enzyme immunoassay method for the analysis of water. It uses a coated tube technology to detect the analyte of concern.

Features:

• The first environmental immunoassay format to be made commercially available
• Broad screen for the triazine family of herbicides including atrazine
• Rapid field method for the analysis of drinking and environmental water samples
• Test up to six tubes at once
• Coated tube immunoassay

Test result type

• Qualitative and Semi-quantitative data

Samples per kit

• 20 tube test kit (tests up to 12 samples)

Assay range

• 0.1 ppb to 1.0 ppb
• LDD = 0.053 ppb (atrazine)

Sample methods

• Water and a variety of other matrices

Sampling time

• Sample-to-data in approximately 10 minutes

Specificity

The EnviroGard® Triazine Test Kit does not differentiate between atrazine and closely related compounds, but detects their presence to differing degrees. The chart below shows the approximate Lower Limits of Detection (LDD) for the cross-reacting compounds.

Basic Test Procedure

• Add 160 uL of calibrator, negative control or sample to the tube
• Add four drops of enzyme conjugate
• Incubate for five minutes
• Decant and wash four times
• Add four drops of substrate and chromogen
• Incubate for two minutes
• Add one drop of stop solution
• Read on photometer

Test kit components

• 20 antibody-coated test tubes
• One vial of negative control
• One vial of atrazine-enzyme conjugate
• One vial of 0.1 ppb atrazine calibrator
• One vial of 1.0 ppb atrazine calibrator
• One vial each substrate and chromagen
• One vial stop solution
• One test tube rack

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use for up to 8 hours
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• Triazine EnviroGard® test kit (7210000)

Required test equipment

• Disposable-tip pipette which will measure 160 uL
• Timer
• Glass tubes or vials for calibrator preparation
• Tap or distilled water for rinsing tubes
• Marker pen
• Pipette that will measure 0.5mL

Other recommended materials

• Calculator
• Spectrophotometer

7311000 EnviroGard Chlordane in Soil Kit by Modern Water

Envirogard® is a rapid field enzyme immunoassay method for the analysis of soil remediation, assessment and industrial testing. It uses a coated tube technology to detect the analyte of concern.

Features:

• Rapid field testing kit
• Semi-quantitative results at set calibrator (action) levels
• Test up to 16 samples at one time
• Results in less than 30 minutes
• Coated tube immunoassay
• Training available
• EPA SW-846 Method #4041

Test result type

• Semi-quantitative data or qualitative

Samples per kit

• Up to 16 soil samples depending on the number of runs and action levels used. (Kit includes 20 antibody coated tubes)

Assay range

• Semi-quantitative results (greater or less than) at calibrator (action) levels of 20, 100 and 600 ppb total Chlordane
• Minimum detection limit (MDL) is 20 ppb Chlordane
• No maximum screening level as extracts can be diluted into test range

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately)
• The sample extraction kit provides material for 12 soil sample extractions with methanol

Sampling time

• Approximately two minutes per sample plus assay run time of approximately 30 minutes

Sampling time

The EnviroGard® Chlordane immunoassay test does not differentiate between Chlordane and other cyclodienes. The table below shows compounds at the minimum detection limit (MDL) - an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross -reactivity values to similar compounds. The approximate Lower Limits of Detection (LDD) for the cross-reacting compounds.

Basic Test Procedure

• Extract the soil sample using the sample extraction kit
• Pipet Chlordane assay diluent into the antibody coated tubes, then add either calibrator or sample extract into the tubes
• Wait for 15 minutes
• Add Chlordane enzyme conjugate to the tubes
• Wait for five minutes, decant and wash the tubes
• Add substrate solution
• Wait for three minutes, add stop solution
• Read the absorbance using a photometer and interpret the results. (Refer to Chlordane Soil test kit instruction guide procedures)
• Stop the reaction and interpret results

Test kit components

• Assay diluent
• 20 antibody-coated test tubes
• Enzyme conjugate, substrate and stop solution reagents
• Calibrators for 20, 100 and 600 ppb Chlordane
• Negative control calibrator
• Test tube rack. and pipet tips
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight
• Portable spectrophotometer battery should be charged prior to use

Required test materials

• EnviroGard Chlordane in soil kit (7311000)
• Sample extraction kit (soil)(7311000EB)

Required test equipment

• Field soil lab (Accessory kit) (6050400) which includes: Repeating pipette*, Adjustable pipette*, Balance*, Repeating pipette tips*, Timer*
• Differential spectrophotometer - 110V (6000001), 220V (6000002)
• Field lab system rental (includes items marked with * above) (6997020)

Other recommended materials

• Latex gloves
• Absorbent paper for blotting test tubes
• Liquid and solid waste containers
• Calculator
• Tap or laboratory grade water for tube rinsing
• Marker pen