Modern Water

AZF686300 Microtox SOLO Reagent, 50 vials per box by Modern Water

SOLO Reagent is the same as Acute Reagent - packaged to run a single test

Accessories required:

• Disposable Glass Cuvettes: box of 1000 - AZF686004
• Cuvette Racks (set of 5) - AZF686250
• Oxford® Fixed Volume 10 uL Pipette (for high toxicity protocol only) - AZF686067
• Adjustable Volume Pipette: 10 - 100 uL - AZF686087
• Adjustable Volume Pipette: 100 - 1000 uL (for all protocols) - A00176
• 10-100 uL Wide Bore Pipette Tips: tray of 96 - AZFV020
• 100-1000 uL Wide Bore Pipette Tips: tray of 100 - AZFV021
• Microtox® Diluent: 1L bottle - AZF686011
• Microtox® Osmotic Adjusting Solution: 1 x 50 mL - AZF686019

ATP Reagent shelf life is 1 year (refrigerated)

AZF848519 Microtox® FX Test System by Modern Water

Microtox® FX is a simple, rapid, extremely responsive, portable water quality test system. Designed for acute toxicity screening and adenosine triphosphate (ATP) testing, Microtox® FX uses bioluminescence technology to screen for contamination in instances of drinking water emergencies and chemical spills into water systems. Microtox® FX is the portable toxicity analyser used with the Microtox® technology

The Microtox® FX instrument has a combined detection capability that provides a very sensitive and rapid test to detect two of the most probable classes of agents; pathogens and toxic chemicals, that may accidently or intentionally contaminate drinking water or wastewater. Microtox® FX's acute toxicity and ATP detection capabilities make it the ideal instrument for rapidly and accurately assessing if the quality of drinking water, from the source to the tap, has been affected by an incident.

• Biological early warning system sensitive to more than 2,700 simple and complex chemicals
• Fast - Reliable results available in 5 minutes after initial sample preparation
• Test results highly correlated with other widely accepted toxicity test methods
• Excellent correlation with HPC test methods
• Fully portable - lightweight with sturdy field carrying case
• Battery life of up to 8 - 10 hours with typical use
• Compatible with widely available ATP test kits*
• Manufactured in a certified ISO 13485 quality system with 100% lot traceability

Microtox® FX is designed for use in any sample location throughout the water distribution or industrial waste water system. It is particularly suited to remote sites such as reservoirs, storage tanks, ocean or lake going vessels or in any hard to reach place.

Chemical contamination of drinking water and wastewater

Microtox® FX is the portable version of the industry-leading Microtox® Model 500 (M500) laboratory analyser. The Microtox® FX test is fast, simple to conduct, uses small sample sizes and is very cost effective. Results correlate well with those from other toxicity bioassays such as fish, daphnia and shrimp. Microtox® FX is used extensively in the measurement of toxicity of "fit for use" water and wastewater treatment effluent. It is also used as an early screening tool for relative toxicity as part of a test battery.

Microtox® FX test systems are uniquely suited for drinking water surveillance where supplies are monitored regularly and at strategic points. It can quickly reveal any changes in the level of toxicity of drinking water making it the ideal solution for major events. Microtox® has been deployed during the Summer Olympics beginning in 1984.

In industrial and municipal wastewater, Microtox® FX helps assure compliance with NPDES toxicity limits, measures toxicity in influent streams and determines treatment efficiency.

Microbial contamination of drinking water

Microtox® FX can rapidly estimate the microbial concentration of a drinking water sample to a level of 100 cfu/mL without additional filtration or incubation steps. The data is available in minutes and is highly correlated with standard heterotrophic plate count methods.

Microtox® FX is extremely responsive and has been designed for use in a wide variety of applications where it is crucial to rapidly determine the biomass of a sample.

Applications

• Drinking water monitoring
• Emergency response: Biological contamination
• Emergency response: Chemical toxicity
• Hazardous waste
• Industrial effluent
• Industrial process water
• Municipal effluent
• Recreational water
• Soil
• Sediments
• Storm water

Process explained

The Microtox® FX system performs a dual function; toxicity testing and determination of microbial contamination. The Microtox® FX test system uses a strain of naturally occurring luminescent bacteria called Aliivibrio fischeri to provide acute toxicity detection. The bacteria emit light as a natural part of their metabolism. Exposure to a toxic substance causes disruption of the respiratory process of the bacteria resulting in reduced light output. The Microtox® FX photometer measures the light levels before and after addition of the sample, and the reduction in light output is a measure of the toxicity of the sample.

All organisms contain ATP as their main energy source and the amount of ATP in a sample is directly proportional to the biomass of the sample.

ATP reacts with luciferin/luciferase, the enzyme system present in firefly tails, to produce light. In the reaction, each molecule of ATP produces one photon of light; the light output of this reaction can be accurately measured using the very sensitive Microtox® FX instrument.

Includes:

• Microtox FX Analyzer, w/Case
• Microtox SOLO Reagent: 50 vials
• Microtox Diluent: 1L
• Microtox Osmotic Adjusting Solution
• Adjustable Volume Pipette, 10-100 uL
• Adjustable Volume Pipette 100-1000 uL
• 10-100 uL Pipette Tips
• 100-1000 uL Pipette tips
• Disposable Glass Cuvettes (400)
• Cuvette Racks: set of 5
• Digital Lab Timer
• User Manual (USB)

A00162 RaPID Assay Total BTEX / TPH 100 Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Results available in approximately 60 minutes
• Training available
• Magnetic particle immunoassay
• Correlated to TPH methods (knowledge of fuel source required)

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples) (Five kit minimum order)

Assay range

• Soil: 0.9 ppm to 30 ppm as total BTEX standard
• Water: 0.02 to 3.0 ppm as total BTEX standard
• asTPH:range varies based on fuel source

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with methanol.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes.

Specificity

The total BTEX/TPH concentrations of the indicated contaminant types in soil samples are expressed below, at each ofthe three kit calibrator levels, in units comparable to results from GC method 8015 (TPH).

The total BTEX/ TPH RaPID assay immunoassay test does not differentiate between BTEX and other petroleum hydrocarbons.The table below shows compounds at the limit of quantitation (LOQ) - an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Zero standard, wash, enzyme conjugate, colour development and stop reagents
• Standards for 0.09, 0.35 and 3.0 ppm as total BTEX
• Kit control as 2.1 ppm as total BTEX
• Disposable test tubes

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• Total BTEX/TPH 100 tube kit (A00162)
• Sample extraction kit(for soil samples only) (A00165EB)
• Total BTEX sample diluent 100ml (as needed to increase range)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-II RaPID analyser (6000111), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

SD50950 QuickChek Sulphate Reducing Bacteria RC II Ten-Pak by Modern Water

The QuickChek™ SRB Detection System is a rapid enzyme immunoassay method that detects sulfate reducing bacteria (SRB). The test employs purified antibodies to detect the enzyme adenosine-5'-phosphosulfonate (APS) reductase which is common to all strains of SRB.

The purified antibodies are attached to small particles that selectively capture the APS reductase enzyme. The particles and captured enzyme are then isolated on a porous membrane, which forms a reactive layer that turns blue in the presence of the APS reductase enzyme.

QuickChek™ SRB offers several advantages over cell culture techniques for SRB detection including immediate and accurate results. The kit allows testing of solid and semi-solid samples and detects all SRB, including SRB that is unable to grow in some standard media. The test results are not compromised by chemical or salinity interferences that are often found in field samples.

The QuickChek™ SRB kit is completely self-contained and disposable. Each kit includes all necessary materials to detect and enumerate sulfate reducing bacteria. There is no need for pretreatment or dilution of samples and there are not special hazardous waste disposal limitations.

Features:

• Semi-quantitative results
• Detects all viable and nonviable SRB samples
• Results available in approximately 8-10 minutes
• Two or three samples simultaneously
• Detection limit of 103 cells/ml
• etection limit for "clean" waters down to 101 cells/ml

Applications

• Corrosion coupons
• Cutting fluids
• Drilling fluids (Muds)
• Solids: biofilms, sludge, rust, mud
• Water associated with oil

SD50951 QuickChek Sulphate Reducing Bacteria RC II Economy-Pak (100) by Modern Water

The QuickChek™ SRB Detection System is a rapid enzyme immunoassay method that detects sulfate reducing bacteria (SRB). The test employs purified antibodies to detect the enzyme adenosine-5'-phosphosulfonate (APS) reductase which is common to all strains of SRB.

The purified antibodies are attached to small particles that selectively capture the APS reductase enzyme. The particles and captured enzyme are then isolated on a porous membrane, which forms a reactive layer that turns blue in the presence of the APS reductase enzyme.

QuickChek™ SRB offers several advantages over cell culture techniques for SRB detection including immediate and accurate results. The kit allows testing of solid and semi-solid samples and detects all SRB, including SRB that is unable to grow in some standard media. The test results are not compromised by chemical or salinity interferences that are often found in field samples.

The QuickChek™ SRB kit is completely self-contained and disposable. Each kit includes all necessary materials to detect and enumerate sulfate reducing bacteria. There is no need for pretreatment or dilution of samples and there are not special hazardous waste disposal limitations.

Features:

• Semi-quantitative results
• Detects all viable and nonviable SRB samples
• Results available in approximately 8-10 minutes
• Two or three samples simultaneously
• Detection limit of 103 cells/ml
• etection limit for "clean" waters down to 101 cells/ml

Applications

• Corrosion coupons
• Cutting fluids
• Drilling fluids (Muds)
• Solids: biofilms, sludge, rust, mud
• Water associated with oil

AZF60A000 Microtox® LX Analyzer (Dual Voltage) with Computer Controller by Modern Water

The new Microtox LX Series is the next generation of laboratory based acute toxicity analyzer. The new analyzer blends Modern Water's proven M500 technology with improved features to simplify testing in demanding drinking, industrial and wastewater applications.

Key Benefits

• Biological earning warning system sensitive to more than 2,700 simple and complex chemicals allows the protection of drinking water supplies from accidental or deliberate contamination.
• Test results highly correlated with other widely accepted toxicity test methods helps to ensure compliance with regulatory and effluent permit standards in wastewater applications.
• Proven - Numerous independent scientific studies have documented Microtox's performance as an effective toxicityscreening tool in a wide array of applications.
• Increased Sensitivity - The use of a new proprietary, fully dynamic photomultiplier increases the sensitivity of the instrument.
• Fast, Reliable and Reproducible Results - Results Available in as little as 15 minutes after initial sample preparation.
• The instrument's new automatic color correction feature adjusts test results based on the sample's turbidity
• Actively cooled sample and read wells enable more precise and consistent readings.
• Cost effective - A low cost toxicity test that requires small sample volumes.
• Manufactured in a certified ISO 13485 quality system with 100% lot traceability

For over 30 years, Modern Water's Microtox technology has provided laboratories with proven, cost effective technology to protect drinking water supplies ensure compliance with regulatory standards and conduct research. The new Microtox LX analyzer builds on the foundation that has made our toxicity product line among the most trusted in the industry.

Applications

Drinking Water Plants

• Monitor for accidental or deliberate contamination
• Provides warning in sufficient time for action
• Rapid screening and confirmatory results
• Check source water
• Check in process water
• Check 'finished' prior to distribution
• Check water distribution system

Wastewater Plants

As part of the pre-treatment program, it allows the facility to:

• Regulate the amount of pollutants coming into the facility
• Maintain smooth operations
• Minimize upsets
• Maintain good compliance performance
• Reduce and control costs and generate revenues by surcharging particularly toxic influent streams

Improve operating efficiency:

• Avoid Unscheduled shutdowns
• Avoid Damage/disruption to biological treatment systems
• Avoid Effluent violations
• Avoid Increasing chemical costs

Microtox Has Been Used In A Broad Range of Applications In A Diverse Range of Industries;

• Drilling Fluids and Drilling Muds
• Mining, wastewater, soil and water
• Industrial Effluents
• Industrial Process Water
• Marine Water
• Medical/Pharmaceutical Products
• Food packaging materials
• Personal care and household chemical analysis
• Sediments
• Storm Water Runoff
• Solid Phase Materials
• Food processing water

7020301 PCB EnviroGard 12T Soil Test Kit by Modern Water

Envirogard® is a rapid field enzyme immunoassay method for the analysis of soil remediation, assessment and industrial testing. It uses a coated tube technology to detect the analyte of concern.

Features:

• Convenient and rapid testing in the field or labatory
• Tests up to 10 samples at once
• Two detection levels of your choice are provided with each sample
• High level of accuracy
• EPA SW-846 Method #4020

Test result type

• Semi-quantitative or qualitative data
• Knowledge of specific aroclor(s) present will aid accuracy

Samples per kit

• Dependent on batch size and number of detection levels
• Each sample can be tested one at a time or in batches of two to ten samples

Assay range

• Soil: 0.5 ppm to 500 ppm with dilutions
• Wipes: 5.0 ug/100cm² to 5000 ug/100cm² with dilutions

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately)
• The sample extraction kit provides materials for 12 soil sample extractions with methanol

Sampling time

• Approximately two minutes per sample plus assay run time of approximately 30 minutes

Basic Test Procedure

• Transfer soil or wipe to extraction jar
• Cap and shake for one minute
• Filter extracted sample
• Pipette and dilute samples with dilution ampule
• Transfer 1 ml of buffer to glass buffer tubes
• Pipette and transfer sample to buffer tubes
• Fit antibody coated tubes on buffer tubes; invert and mix;return to antibody coated tube
• Incubate for 10 minutes
• Connect antibody coated and conjugate tubes; invert and mix, return to antibody coated tube
• Incubate five minutes
• Wash antibody coated tubes vigorously four times
• Using a repeater pipette at setting two and 5 mL tip,transfer 200 uL substrateA to each tube
• Add 200 uL substrate B to each tube and mix
• Inclubate for 2.5 minutes
• Add 200 uL STOP to each tube
• Read tubes in photometer.Record data

Test kit components

• 48 antibody coated tubes and PCB enzyme conjugate vials
• 48 glass buffer tubes
• Substrate and stop solution reagents
• PCB standard
• Pipette tips and bulb pipettes
• Buffer
• Amber vials
• Wash solution
• 12 ampule crackers

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging.
• Store at ambient temperature 64° to 81°F (18° to 27°C)
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not freeze test kit components

Required test materials

• PCB EnviroGard 12T accessory kit (6050400)
• PCB EnviroGard 12T accessory kit rental (Includes all equipment required to operate test and interpret results)(6997020)

Required test equipment

• PCB EnviroGard 12T soil test kit (7020301)
• PCB soil sample extraction kit (7020301EB)
• EnviroGard PCB wipe sample extraction kit (7020301WA)

Other recommended materials

• Absorbent paper
• Liquid waste container
• Disposable gloves
• Marker pen
• Liquid and solid waste containers

Will last for approximately 2 boxes of reagent or 20 vials; nontoxic, 2% sodium chloride solution; shelf life is one year at RT.

Vibrio fischeri bacteria; shelf life is 18 months when stored at (-20C) - (-25C); on average one vial will provide enough reagent for 20-25 tests (depending on number of dilutions, test protocols, controls, etc.)

A00205 RaPID Assay Carcinogenic PAHs (CPAH) 100 Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Test up to 50 samples at one time
• Results available in approximately 60 minutes
• Training available
• Magnetic particle immunoassay
• EPA SW-846 method #4035 with increased sensitivity to carcinogenic PAHs

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• Soil: 10 ppb to 500 ppb as Benzo(a)pyrene
• Water: 0.1 to 5.0 ppb as Benzo(a)pyrene
• Range can be extended with additional dilutions
• Site specific calibration required to meet total PAH/C-PAH analysis requirements

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with methanol.
• Water samples must be diluted with an equal volume of methanol to prevent adsorptive loss.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes

Specificity

The total Carcinogenic PAH concentrations (sum of seven Carcinogenic PAH compounds) of the indicated contaminant types in soil samples are expressed below, at each of the three kit calibrator levels, in units comparable to results from GC method 8015 (TPH).

The RaPID assay Carcinogenic PAH immunoassay test does not differentiate between benzo(a)pyrene and other polyaromatic hydrocarbons. The table below shows compounds at the limit of quantitation (LOQ)- an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Test kit components

• Antibody coated magnetic particles, enzyme conjugate, colour development and stop and wash solutions for analysis of 100 test tubes
• Standards for 0.1, 1.0 and 5.0 ppb as Benzo(a) pyrene
• Kit control as 2.0 ppb as Benzo(a)pyrene
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• CPAH 100 tube kit (A00205)
• Sample extraction kit(for soil samples only) (A00204EA)
• cPAH sample diluent (100 mL) (A00203)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-II analyser (6000081), Printer (6000082), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips(12.5 mL) (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

A00134 RaPID Assay PCBs 100 Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Results available in approximately 60 minutes
• Training available
• Magnetic particle immunoassay
• EPA SW-846 method #4020

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• Soil: 0.5 ppm to 10 ppm total PCB's as Aroclor 1254
• Water: 0.25 to 5.0 ppb total PCB s as Aroclor 1254
• Wipes: 5 to 100μg per wipe total PCB s as Aroclor 1254
• Range can be extended with additional dilutions

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with methanol.
• Water samples must be diluted one part sample to one part methanol to prevent adsorptive loss.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes

Specificity

The table below shows compounds at the method detection limit(MDL) which isthe lowest concentration of the compound that can be picked up in the assay. The limit of quantification (LOQ)- an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Basic Test Procedure

• Add calibrators and sample, enzyme conjugate, and antibody coupled magnetic particles to a test tube. Vortex.
• Incubate for 15 minutes
• Using the RaPID magnetic separator, decant and wash two times
• Add colour solution and incubate for 20 minutes
• Stop the reaction and read colour at 450 nm
• Quantitive results and QC parameters are calculated and printed automatically using the RPA spectrophotometer

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Zero standard, wash, enzyme conjugate, colour development and stop reagents
• Standards for 0.25, 1.00 and 5.0 ppb as Aroclor 1254
• Kit control as 3.0 ppb as Aroclor 1254
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• PCB Assay 100 tube kit (A00134)
• Sample extraction kit(for soil samples only) (A00137EB)
• Sample extraction kit(for wipe samples only) (A00137WB)
• PCB sample diluent 100mL (A00136)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-I1 RaPID analyser (6000081), Printer (6000082), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips(12.5 mL) (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

A00157 RaPID Assay PAHs 100 Test Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Results available in approximately 60 minutes
• Tests up to 50 samples at once
• Magnetic particle immunoassay
• Training available
• EPA SW-846 method #4035

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• Soil: 0.2 ppm to 5 ppm as Phenanthrene
• Water: 2.66ppb to 66.5ppb as Phenanthrene
• Range can be extended with additional dilutions
• Site specific calibration required to meet total PAH analysis

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions
• Water samples must be diluted three parts sample to one part methanol to prevent adsorptive loss

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes

Specificity

The total PAHs (sum of 16 PAH compounds) of the indicated contaminant types in soil samples are expressed below, at each of the three kit calibrator (standards) levels, in units comparable to results from GC method 8270 or HPLC method 8310.

The RaPIDAssay® PAH immunoassay test does not differentiate between phenanthrene and other polyaromatic hydrocarbons.The table below shows compounds at the limit of quantitation (LOQ) - an approximate concentration required to yield a positive result at the lowest standard.The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Diluent zero, wash, enzyme conjugate, colour development, stop reagents and wash solution
• Standards for 2, 10 and 50 ppb as Phenanthrene
• Kit control as 25 ppb as Phenanthrene
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• PAH 100 test tube kit (A00157)
• Sample extraction kit(for soil samples only) (A00160EA)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-II RaPID analyser (6000081), Printer (6000082), Magnetic separation rack (A00004C), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

A00071 Atrazine 100 Test Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil, food or water samples
• Quantitative data results with excellent analytical precision
• Results available in approximately 60 minutes
• Tests up to 50 samples at once
• Magnetic particle immunoassay
• Training available
• EPA SW-846 method #4670 for drinking water

Test result type

• Quantitative, semi-quantitative or qualitative depending on protocol selected

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• 0.04 to 5.0 ppb
• LDD = 0.1 ppb

Sample methods

• Methods exist for soil and a wide variety of commodities

Sampling time

• 'Sample-to-Data' in approximately 60 minutes

Specificity

Basic Test Procedure

• Add 200 μL of calibrator or prepared sample, 250μL enzyme conjugate and 500 μL antibody coupled magnetic particles.Vortex
• Incubate for 15 minutes
• Using the RaPID magnetic separator, decant and wash
• Add 500 μL colour solution
• Incubate for 20 minutes
• Stop the reaction and read colour at 450 nm
• Quantitive results and QC parameters are calculated and printed automatically using the RPA-II® spectrophotometer

Test kit components

• Antibody coated magnetic particles, enzyme conjugate, colour development, stop andwash reagents for analysis of 100 test tubes
• Standards for 0.1 1.0 and 5.0 ppb Atrazine
• Zero standard and 3.0 ppb control standard
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• Atrazine RaPID Assay (A00071)
• Atrazine sample diluent (100mL) (A00051)

Required test equipment

• Accessory kit (purchase) (6050100)
• Accessory kit (rental) (6997010)
• Accessory kit components: Magnetic separation rack (A00004), Repeator pipette (A00008), Adjustable volume pipette (A00176), Adjustable volume pipette (6000111), RPA-II analyser with printer (A00014), Vortex mixer (A00014), Digital timer (A00015)

AZF686060 Microtox Acute Starter Pack by Modern Water

Only available with purchase of M500 analyser. Includes the following:

• 1 AZF686018 Microtox® Acute Reagent 10 vials
• 1 AZF686011 Microtox® Diluent
• 1 AZF686019 Microtox® Osmotic Adjusting Sol'n
• 2 AZF686016 Microtox® Reconstitution Solution
• 1 686005 Disposable Glass Cuvettes: 400x

7540000 EnviroGard Microcystins Plate Kit by Modern Water

Envirogard® is a rapid laboratory enzyme immunoassay method for the analysis of water. It uses a plate technology to detect the analyte of concern.

Features:

• Rapid lab testing method for the detection of microcystins and nodularins in drinking and environmental water samples
• Conforms to ISO standards for environmental immunoassays such as ISO 15089
• Quantiative data results with excellent analytical precision
• Test up to 88 samples* at once for increased cost efficiency
• Results available in approximately 120 minutes
• Microtiter plate immunoassay
• Ideal for source water monitoring

Test result type

• Quantitative data

Samples per kit

• 96 well test kit (tests up to 88 samples*)

Assay range

• 0.1 ppb to 1.6 ppb
• Extended range available

Sampling time

• Sample-to-data in approximately 120 minutes

Specificity

The proprietary antibody used in this assay detects microcystins and nodularins. The crossreactivity of the Microcycstins EnviroGard® Plate Kit for various microcystin variants is shown below at 50% B/B₀

Basic Test Procedure

• Prepare standards
• Add 100 uL of sample or standard
• Incubate for 30 minutes
• Add 100 uL enzyme conjugate
• Incubate for 30 minutes
• Decant and wash five times
• Add 100 uL colour solution
• Incubate for 30 minutes
• Stop the reaction and read colour at 450nm

Test kit components

• Antibody coated microtiter plate strips, enzyme conjugate, colour development and stop reagent for analysis of 96 wells
• One vial each of 0.1, 0.2, 0.4, 0.56, 0.8 and 1.6 ppb microcystin-LR surrogate (nontoxic) calibrators
• Test kit instructions
• Optional 1.5 ppb check standard

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use for up to eight hours
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• Microcystins EnviroGard® plate (7540000)

Required test equipment

• 10 cc syringes
• 0.45 micron non-sterile filter units
• Pipette to deliver 100 mL
• Distilled or DI water for rinsing wells
• Microtiter plate reder or stip reader
• Timer
• Parafilm or tape
• Marker pen

Other recommended materials

• Orbital shaker
• Multi-channel pipette
• Repeater pipette
• Calculator
• Microtiter plate washer
• Solid extraction devices