$500 - $750, EnviroGard�, Modern Water

7540000 EnviroGard Microcystins Plate Kit by Modern Water

Envirogard® is a rapid laboratory enzyme immunoassay method for the analysis of water. It uses a plate technology to detect the analyte of concern.

Features:

• Rapid lab testing method for the detection of microcystins and nodularins in drinking and environmental water samples
• Conforms to ISO standards for environmental immunoassays such as ISO 15089
• Quantiative data results with excellent analytical precision
• Test up to 88 samples* at once for increased cost efficiency
• Results available in approximately 120 minutes
• Microtiter plate immunoassay
• Ideal for source water monitoring

Test result type

• Quantitative data

Samples per kit

• 96 well test kit (tests up to 88 samples*)

Assay range

• 0.1 ppb to 1.6 ppb
• Extended range available

Sampling time

• Sample-to-data in approximately 120 minutes

Specificity

The proprietary antibody used in this assay detects microcystins and nodularins. The crossreactivity of the Microcycstins EnviroGard® Plate Kit for various microcystin variants is shown below at 50% B/B₀

Basic Test Procedure

• Prepare standards
• Add 100 uL of sample or standard
• Incubate for 30 minutes
• Add 100 uL enzyme conjugate
• Incubate for 30 minutes
• Decant and wash five times
• Add 100 uL colour solution
• Incubate for 30 minutes
• Stop the reaction and read colour at 450nm

Test kit components

• Antibody coated microtiter plate strips, enzyme conjugate, colour development and stop reagent for analysis of 96 wells
• One vial each of 0.1, 0.2, 0.4, 0.56, 0.8 and 1.6 ppb microcystin-LR surrogate (nontoxic) calibrators
• Test kit instructions
• Optional 1.5 ppb check standard

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use for up to eight hours
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• Microcystins EnviroGard® plate (7540000)

Required test equipment

• 10 cc syringes
• 0.45 micron non-sterile filter units
• Pipette to deliver 100 mL
• Distilled or DI water for rinsing wells
• Microtiter plate reder or stip reader
• Timer
• Parafilm or tape
• Marker pen

Other recommended materials

• Orbital shaker
• Multi-channel pipette
• Repeater pipette
• Calculator
• Microtiter plate washer
• Solid extraction devices

7210000 EnviroGard Triazine Test Kit by Modern Water

Envirogard® is a rapid field enzyme immunoassay method for the analysis of water. It uses a coated tube technology to detect the analyte of concern.

Features:

• The first environmental immunoassay format to be made commercially available
• Broad screen for the triazine family of herbicides including atrazine
• Rapid field method for the analysis of drinking and environmental water samples
• Test up to six tubes at once
• Coated tube immunoassay

Test result type

• Qualitative and Semi-quantitative data

Samples per kit

• 20 tube test kit (tests up to 12 samples)

Assay range

• 0.1 ppb to 1.0 ppb
• LDD = 0.053 ppb (atrazine)

Sample methods

• Water and a variety of other matrices

Sampling time

• Sample-to-data in approximately 10 minutes

Specificity

The EnviroGard® Triazine Test Kit does not differentiate between atrazine and closely related compounds, but detects their presence to differing degrees. The chart below shows the approximate Lower Limits of Detection (LDD) for the cross-reacting compounds.

Basic Test Procedure

• Add 160 uL of calibrator, negative control or sample to the tube
• Add four drops of enzyme conjugate
• Incubate for five minutes
• Decant and wash four times
• Add four drops of substrate and chromogen
• Incubate for two minutes
• Add one drop of stop solution
• Read on photometer

Test kit components

• 20 antibody-coated test tubes
• One vial of negative control
• One vial of atrazine-enzyme conjugate
• One vial of 0.1 ppb atrazine calibrator
• One vial of 1.0 ppb atrazine calibrator
• One vial each substrate and chromagen
• One vial stop solution
• One test tube rack

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use for up to 8 hours
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• Triazine EnviroGard® test kit (7210000)

Required test equipment

• Disposable-tip pipette which will measure 160 uL
• Timer
• Glass tubes or vials for calibrator preparation
• Tap or distilled water for rinsing tubes
• Marker pen
• Pipette that will measure 0.5mL

Other recommended materials

• Calculator
• Spectrophotometer