Rapid Assay Accessory Kit

A00157 RaPID Assay PAHs 100 Test Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Results available in approximately 60 minutes
• Tests up to 50 samples at once
• Magnetic particle immunoassay
• Training available
• EPA SW-846 method #4035

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• Soil: 0.2 ppm to 5 ppm as Phenanthrene
• Water: 2.66ppb to 66.5ppb as Phenanthrene
• Range can be extended with additional dilutions
• Site specific calibration required to meet total PAH analysis

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions
• Water samples must be diluted three parts sample to one part methanol to prevent adsorptive loss

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes

Specificity

The total PAHs (sum of 16 PAH compounds) of the indicated contaminant types in soil samples are expressed below, at each of the three kit calibrator (standards) levels, in units comparable to results from GC method 8270 or HPLC method 8310.

The RaPIDAssay® PAH immunoassay test does not differentiate between phenanthrene and other polyaromatic hydrocarbons.The table below shows compounds at the limit of quantitation (LOQ) - an approximate concentration required to yield a positive result at the lowest standard.The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Diluent zero, wash, enzyme conjugate, colour development, stop reagents and wash solution
• Standards for 2, 10 and 50 ppb as Phenanthrene
• Kit control as 25 ppb as Phenanthrene
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• PAH 100 test tube kit (A00157)
• Sample extraction kit(for soil samples only) (A00160EA)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-II RaPID analyser (6000081), Printer (6000082), Magnetic separation rack (A00004C), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

A00134 RaPID Assay PCBs 100 Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Results available in approximately 60 minutes
• Training available
• Magnetic particle immunoassay
• EPA SW-846 method #4020

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• Soil: 0.5 ppm to 10 ppm total PCB's as Aroclor 1254
• Water: 0.25 to 5.0 ppb total PCB s as Aroclor 1254
• Wipes: 5 to 100μg per wipe total PCB s as Aroclor 1254
• Range can be extended with additional dilutions

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with methanol.
• Water samples must be diluted one part sample to one part methanol to prevent adsorptive loss.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes

Specificity

The table below shows compounds at the method detection limit(MDL) which isthe lowest concentration of the compound that can be picked up in the assay. The limit of quantification (LOQ)- an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Basic Test Procedure

• Add calibrators and sample, enzyme conjugate, and antibody coupled magnetic particles to a test tube. Vortex.
• Incubate for 15 minutes
• Using the RaPID magnetic separator, decant and wash two times
• Add colour solution and incubate for 20 minutes
• Stop the reaction and read colour at 450 nm
• Quantitive results and QC parameters are calculated and printed automatically using the RPA spectrophotometer

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Zero standard, wash, enzyme conjugate, colour development and stop reagents
• Standards for 0.25, 1.00 and 5.0 ppb as Aroclor 1254
• Kit control as 3.0 ppb as Aroclor 1254
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• PCB Assay 100 tube kit (A00134)
• Sample extraction kit(for soil samples only) (A00137EB)
• Sample extraction kit(for wipe samples only) (A00137WB)
• PCB sample diluent 100mL (A00136)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-I1 RaPID analyser (6000081), Printer (6000082), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips(12.5 mL) (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

A00111 RaPID Assay Pentachlorophenol (PCP) 100 Tube Test Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Tests up to 50 samples at once
• Results available in approximately 60 minutes
• Magnetic particle immunoassay
• Training available
• EPA SW-846 method #4010

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80 samples)

Assay range

• Soil: 0.1 ppm to 10 ppm as pentachlorophenol
• Water: 0.1 to 10.0 ppb as pentachlorophenol
• Other applications are also available
• Range can be extended with additional dilutions

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with alkaline methanol.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes.

Specificity

The PCP RaPID Assay® immunoassay test does not differentiate between PCP and other organochlorines. The table below shows compounds at the limit of quantitation (LOQ)- an approximate concentration required to yield a positive result at the lowest standard. The IC50 isthe concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Basic Test Procedure

• Add prepared sample, enzyme conjugate, and antibody coupled magnetic particles.Vortex
• Incubate for 30 minutes
• Separate using the RaPID magnetic separator. decant and wash
• Add colour solution and incubate for 20 minutes
• Stop the reaction and read colour at 450 nm
• Quantitive results and QC parameters are calculated and printed automatically using the RPA-1I spectrophotometer

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Zero standard, wash, enzyme conjugate, colour development and stop reagents
• Standards for 0.1, 2 and 10 ppb as PCP
• Kit control as 1.0 ppb as PCP
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) for up to eight hours
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight
• Portable spectrophotometer battery should be charged prior to use

Required test materials

• PCP 100 tube test kit (A00111)
• Sample extraction kit (A00128EA)
• PCP sample diluent(as needed) (A00113)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-I1 RaPID analyser (6000111), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

A00162 RaPID Assay Total BTEX / TPH 100 Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Results available in approximately 60 minutes
• Training available
• Magnetic particle immunoassay
• Correlated to TPH methods (knowledge of fuel source required)

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples) (Five kit minimum order)

Assay range

• Soil: 0.9 ppm to 30 ppm as total BTEX standard
• Water: 0.02 to 3.0 ppm as total BTEX standard
• asTPH:range varies based on fuel source

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with methanol.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes.

Specificity

The total BTEX/TPH concentrations of the indicated contaminant types in soil samples are expressed below, at each ofthe three kit calibrator levels, in units comparable to results from GC method 8015 (TPH).

The total BTEX/ TPH RaPID assay immunoassay test does not differentiate between BTEX and other petroleum hydrocarbons.The table below shows compounds at the limit of quantitation (LOQ) - an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Zero standard, wash, enzyme conjugate, colour development and stop reagents
• Standards for 0.09, 0.35 and 3.0 ppm as total BTEX
• Kit control as 2.1 ppm as total BTEX
• Disposable test tubes

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• Total BTEX/TPH 100 tube kit (A00162)
• Sample extraction kit(for soil samples only) (A00165EB)
• Total BTEX sample diluent 100ml (as needed to increase range)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-II RaPID analyser (6000111), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

A00205 RaPID Assay Carcinogenic PAHs (CPAH) 100 Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Test up to 50 samples at one time
• Results available in approximately 60 minutes
• Training available
• Magnetic particle immunoassay
• EPA SW-846 method #4035 with increased sensitivity to carcinogenic PAHs

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• Soil: 10 ppb to 500 ppb as Benzo(a)pyrene
• Water: 0.1 to 5.0 ppb as Benzo(a)pyrene
• Range can be extended with additional dilutions
• Site specific calibration required to meet total PAH/C-PAH analysis requirements

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with methanol.
• Water samples must be diluted with an equal volume of methanol to prevent adsorptive loss.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes

Specificity

The total Carcinogenic PAH concentrations (sum of seven Carcinogenic PAH compounds) of the indicated contaminant types in soil samples are expressed below, at each of the three kit calibrator levels, in units comparable to results from GC method 8015 (TPH).

The RaPID assay Carcinogenic PAH immunoassay test does not differentiate between benzo(a)pyrene and other polyaromatic hydrocarbons. The table below shows compounds at the limit of quantitation (LOQ)- an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Test kit components

• Antibody coated magnetic particles, enzyme conjugate, colour development and stop and wash solutions for analysis of 100 test tubes
• Standards for 0.1, 1.0 and 5.0 ppb as Benzo(a) pyrene
• Kit control as 2.0 ppb as Benzo(a)pyrene
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• CPAH 100 tube kit (A00205)
• Sample extraction kit(for soil samples only) (A00204EA)
• cPAH sample diluent (100 mL) (A00203)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-II analyser (6000081), Printer (6000082), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips(12.5 mL) (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

A00082 2, 4-D 100 Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Test up to 50 samples at one time
• Results available in approximately 60 minutes
• Training available
• Magnetic particle immunoassay
• EPA SW-846 method #4015

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• Soil: 0.15 ppm to 7.5 ppm as 2, 4-D
• Water: 1.0 to 50.0 ppb as 2, 4-D
• Range can be extended with additional dilutions

Sample preparation

• Soil samples require prior extraction as indicated in the application procedure

Sampling time

• A 16 hour extraction time is recommended for soil Method 4015

Specificity

The 2, 4D RaPID Assay immunoassay test does not differentiate between 2,4-D and other chlorophenoxy acids. The table below shows compounds at the limit of quantitation (LOQ) -an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the color produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Basic Test Procedure

• Add standards, prepared sample, enzyme conjugate, and antibody coupled magnetic particles.Vortex.
• Incubate for 30 minutes
• Using the magnetic separator, decant and wash
• Add colour solution, incubate 20 minutes
• Stop the reaction and read colour at 450 nm
• Quantitative results and QC parameters are calculated and printed automatically using the RPA-1I spectrophotometer

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Zero standard, wash, enzyme conjugate, colour development and stop reagents
• Standards for 1, 10 and 50 ppb as 2, 4-D
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use for up to 8 hours
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• 2, 4-D 100 tube kit (A00082)
• 2, 4-D sample diluent (A00079)

Required test equipment

• RaPID Assay accessory kit (6050100 (P)/6997010 (R)) which contains: RPAI1 analyser (6000081), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips (12.5 mL) (A00013)
• Adjustable pipet tips

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

7002000 EnSys TNT Explosives Soil Test Kit by Modern Water

EnSys® explosives kits are rapid field or laboratory colourimetric tests for the analysis of explosives in soil. The kits are based on a method developed by Dr. Thomas Jenkins at the US Army Corps of Engineers - CRREL.

Features:

• Detects TNT, DNT and related explosives
• Convenient and rapid testing in the field or laboratory
• Extractions can be performed simultaneously with analysis in singlicate
• TNT extracts can be used with EnSys RDX test
• Training available
• EPA SW-846 Method #8515

Test result type

• Quantitative data

Samples per kit

• 20 soil samples

Assay range

• Soil: 1 ppm to 30 ppm total TNT
• Higher sample concentrations can be quantified by sample extract dilution

Sample preparation

• Soil samples require prior extraction using the included extraction components
• Soil samples should be dried prior to analysis
• Soil sample extracts may be saved for use with the RDXsoil test kit

Sampling time

• 'Dirt-to-Data' in approximately 30 minutes
• Typically about ten samples can be run in about 40 minutes
• Soil extraction time is typically 2 - 10 minutes per sample plus test run time of approximately 2 minutes

Specificity

The EnSys® TNT test is specific for TNT and related explosive compounds and exhibits the following sensitivities. The TNT test does not measure RDX or HMX.

Basic Test Procedure

• Clean cuvettes and set spectrophotometer
• Extract soil sample: Weigh 10 grams of soil sample, Add sample to extraction jar, Measure 50mL acetone into 50mL tube, Pour acetone into extraction jar and shake for three minutes, Allow to settle for five minutes
• Draw 25mL liquid into the syringe from above the sediment layer in extraction jar
• Attach filter tip to syringe and transfer 25mL of sample extract into a cuvette.
• Place cuvette in spectrophotometer and record initial absorbance
• Add one drop of developer solution, cap and shake for three seconds.
• Remove stopper and place the'sample' cuvette in cell holder and read sample absorbance.
• NOTE: For analysis of samples containing DNT,and/or where DNT concentration is of concern, samples must be allowed to develop for 10 minutes before reading sample absorbance. This will not affect colour development for other nitroaromatics.
• Record data and calculate results.

Test kit components

• 20 weigh boats and wooden spatulas
• 20 extraction jars
• One 50mL graduated tube
• 20 - 30cc syringes and syringe filters
• 1 bulb pipette
• 1 TNT control vial and ampoule cracker
• Developer solution
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging.
• Store at ambient temperature 64° to 81°F (18° to 27°C)
• Operate the test at temperature greater than 40°F/4°C and less than 100°F/39°C (4°C)

Required test materials

• EnSys TNT explosives soil test kit (7002000)

Required test equipment

• EnSys TNT accessory kit (rental, includes HACH DR2800) (6999700)
• Acetone: hardware or laboratory grade (min 50mL per sample)

Other recommended materials

• Hach DR/2000, DR/2010 or DR/2800
• 2 matched hach cuvettes
• 2 cuvette stopper plugs
• Balance
• Tap or laboratory grade water for cuvette rinsing
• Marker pen, calculator
• Absorbent paper
• Liquid waste container
• PPE: Disposable gloves, eyewear