Modern Water, Test and Measurement

A00205 RaPID Assay Carcinogenic PAHs (CPAH) 100 Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Test up to 50 samples at one time
• Results available in approximately 60 minutes
• Training available
• Magnetic particle immunoassay
• EPA SW-846 method #4035 with increased sensitivity to carcinogenic PAHs

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• Soil: 10 ppb to 500 ppb as Benzo(a)pyrene
• Water: 0.1 to 5.0 ppb as Benzo(a)pyrene
• Range can be extended with additional dilutions
• Site specific calibration required to meet total PAH/C-PAH analysis requirements

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with methanol.
• Water samples must be diluted with an equal volume of methanol to prevent adsorptive loss.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes

Specificity

The total Carcinogenic PAH concentrations (sum of seven Carcinogenic PAH compounds) of the indicated contaminant types in soil samples are expressed below, at each of the three kit calibrator levels, in units comparable to results from GC method 8015 (TPH).

The RaPID assay Carcinogenic PAH immunoassay test does not differentiate between benzo(a)pyrene and other polyaromatic hydrocarbons. The table below shows compounds at the limit of quantitation (LOQ)- an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Test kit components

• Antibody coated magnetic particles, enzyme conjugate, colour development and stop and wash solutions for analysis of 100 test tubes
• Standards for 0.1, 1.0 and 5.0 ppb as Benzo(a) pyrene
• Kit control as 2.0 ppb as Benzo(a)pyrene
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• CPAH 100 tube kit (A00205)
• Sample extraction kit(for soil samples only) (A00204EA)
• cPAH sample diluent (100 mL) (A00203)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-II analyser (6000081), Printer (6000082), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips(12.5 mL) (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

AZF686060 Microtox Acute Starter Pack by Modern Water

Only available with purchase of M500 analyser. Includes the following:

• 1 AZF686018 Microtox® Acute Reagent 10 vials
• 1 AZF686011 Microtox® Diluent
• 1 AZF686019 Microtox® Osmotic Adjusting Sol'n
• 2 AZF686016 Microtox® Reconstitution Solution
• 1 686005 Disposable Glass Cuvettes: 400x

A00111 RaPID Assay Pentachlorophenol (PCP) 100 Tube Test Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Tests up to 50 samples at once
• Results available in approximately 60 minutes
• Magnetic particle immunoassay
• Training available
• EPA SW-846 method #4010

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80 samples)

Assay range

• Soil: 0.1 ppm to 10 ppm as pentachlorophenol
• Water: 0.1 to 10.0 ppb as pentachlorophenol
• Other applications are also available
• Range can be extended with additional dilutions

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with alkaline methanol.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes.

Specificity

The PCP RaPID Assay® immunoassay test does not differentiate between PCP and other organochlorines. The table below shows compounds at the limit of quantitation (LOQ)- an approximate concentration required to yield a positive result at the lowest standard. The IC50 isthe concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Basic Test Procedure

• Add prepared sample, enzyme conjugate, and antibody coupled magnetic particles.Vortex
• Incubate for 30 minutes
• Separate using the RaPID magnetic separator. decant and wash
• Add colour solution and incubate for 20 minutes
• Stop the reaction and read colour at 450 nm
• Quantitive results and QC parameters are calculated and printed automatically using the RPA-1I spectrophotometer

Test kit components

• Antibody coated magnetic particles for analysis of 100 test tubes
• Zero standard, wash, enzyme conjugate, colour development and stop reagents
• Standards for 0.1, 2 and 10 ppb as PCP
• Kit control as 1.0 ppb as PCP
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) for up to eight hours
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight
• Portable spectrophotometer battery should be charged prior to use

Required test materials

• PCP 100 tube test kit (A00111)
• Sample extraction kit (A00128EA)
• PCP sample diluent(as needed) (A00113)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-I1 RaPID analyser (6000111), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

6050400 EnSys/EnviroGard Field Accessory Kit by Modern Water

Includes:

• Artel Differential Photometer (1)
• Eppendorff Repeating Pipettor (1)
• 50 mL Repeating Pipettor Tips (1)
• 5 mL Repeating Pipettor Tips (3)
• 12.5 mL Repeating Pipettor Tips (6)
• 12x75 mm polystyrene Test Tubes (6)
• Digital Balance
• Microman Positive
• Displacement Pipettor (1)
• Wash Bottle (1)
• Digital Timer (1)
• Workstation (1)

7020301 PCB EnviroGard 12T Soil Test Kit by Modern Water

Envirogard® is a rapid field enzyme immunoassay method for the analysis of soil remediation, assessment and industrial testing. It uses a coated tube technology to detect the analyte of concern.

Features:

• Convenient and rapid testing in the field or labatory
• Tests up to 10 samples at once
• Two detection levels of your choice are provided with each sample
• High level of accuracy
• EPA SW-846 Method #4020

Test result type

• Semi-quantitative or qualitative data
• Knowledge of specific aroclor(s) present will aid accuracy

Samples per kit

• Dependent on batch size and number of detection levels
• Each sample can be tested one at a time or in batches of two to ten samples

Assay range

• Soil: 0.5 ppm to 500 ppm with dilutions
• Wipes: 5.0 ug/100cm² to 5000 ug/100cm² with dilutions

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately)
• The sample extraction kit provides materials for 12 soil sample extractions with methanol

Sampling time

• Approximately two minutes per sample plus assay run time of approximately 30 minutes

Basic Test Procedure

• Transfer soil or wipe to extraction jar
• Cap and shake for one minute
• Filter extracted sample
• Pipette and dilute samples with dilution ampule
• Transfer 1 ml of buffer to glass buffer tubes
• Pipette and transfer sample to buffer tubes
• Fit antibody coated tubes on buffer tubes; invert and mix;return to antibody coated tube
• Incubate for 10 minutes
• Connect antibody coated and conjugate tubes; invert and mix, return to antibody coated tube
• Incubate five minutes
• Wash antibody coated tubes vigorously four times
• Using a repeater pipette at setting two and 5 mL tip,transfer 200 uL substrateA to each tube
• Add 200 uL substrate B to each tube and mix
• Inclubate for 2.5 minutes
• Add 200 uL STOP to each tube
• Read tubes in photometer.Record data

Test kit components

• 48 antibody coated tubes and PCB enzyme conjugate vials
• 48 glass buffer tubes
• Substrate and stop solution reagents
• PCB standard
• Pipette tips and bulb pipettes
• Buffer
• Amber vials
• Wash solution
• 12 ampule crackers

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging.
• Store at ambient temperature 64° to 81°F (18° to 27°C)
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not freeze test kit components

Required test materials

• PCB EnviroGard 12T accessory kit (6050400)
• PCB EnviroGard 12T accessory kit rental (Includes all equipment required to operate test and interpret results)(6997020)

Required test equipment

• PCB EnviroGard 12T soil test kit (7020301)
• PCB soil sample extraction kit (7020301EB)
• EnviroGard PCB wipe sample extraction kit (7020301WA)

Other recommended materials

• Absorbent paper
• Liquid waste container
• Disposable gloves
• Marker pen
• Liquid and solid waste containers

7002000 EnSys TNT Explosives Soil Test Kit by Modern Water

EnSys® explosives kits are rapid field or laboratory colourimetric tests for the analysis of explosives in soil. The kits are based on a method developed by Dr. Thomas Jenkins at the US Army Corps of Engineers - CRREL.

Features:

• Detects TNT, DNT and related explosives
• Convenient and rapid testing in the field or laboratory
• Extractions can be performed simultaneously with analysis in singlicate
• TNT extracts can be used with EnSys RDX test
• Training available
• EPA SW-846 Method #8515

Test result type

• Quantitative data

Samples per kit

• 20 soil samples

Assay range

• Soil: 1 ppm to 30 ppm total TNT
• Higher sample concentrations can be quantified by sample extract dilution

Sample preparation

• Soil samples require prior extraction using the included extraction components
• Soil samples should be dried prior to analysis
• Soil sample extracts may be saved for use with the RDXsoil test kit

Sampling time

• 'Dirt-to-Data' in approximately 30 minutes
• Typically about ten samples can be run in about 40 minutes
• Soil extraction time is typically 2 - 10 minutes per sample plus test run time of approximately 2 minutes

Specificity

The EnSys® TNT test is specific for TNT and related explosive compounds and exhibits the following sensitivities. The TNT test does not measure RDX or HMX.

Basic Test Procedure

• Clean cuvettes and set spectrophotometer
• Extract soil sample: Weigh 10 grams of soil sample, Add sample to extraction jar, Measure 50mL acetone into 50mL tube, Pour acetone into extraction jar and shake for three minutes, Allow to settle for five minutes
• Draw 25mL liquid into the syringe from above the sediment layer in extraction jar
• Attach filter tip to syringe and transfer 25mL of sample extract into a cuvette.
• Place cuvette in spectrophotometer and record initial absorbance
• Add one drop of developer solution, cap and shake for three seconds.
• Remove stopper and place the'sample' cuvette in cell holder and read sample absorbance.
• NOTE: For analysis of samples containing DNT,and/or where DNT concentration is of concern, samples must be allowed to develop for 10 minutes before reading sample absorbance. This will not affect colour development for other nitroaromatics.
• Record data and calculate results.

Test kit components

• 20 weigh boats and wooden spatulas
• 20 extraction jars
• One 50mL graduated tube
• 20 - 30cc syringes and syringe filters
• 1 bulb pipette
• 1 TNT control vial and ampoule cracker
• Developer solution
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging.
• Store at ambient temperature 64° to 81°F (18° to 27°C)
• Operate the test at temperature greater than 40°F/4°C and less than 100°F/39°C (4°C)

Required test materials

• EnSys TNT explosives soil test kit (7002000)

Required test equipment

• EnSys TNT accessory kit (rental, includes HACH DR2800) (6999700)
• Acetone: hardware or laboratory grade (min 50mL per sample)

Other recommended materials

• Hach DR/2000, DR/2010 or DR/2800
• 2 matched hach cuvettes
• 2 cuvette stopper plugs
• Balance
• Tap or laboratory grade water for cuvette rinsing
• Marker pen, calculator
• Absorbent paper
• Liquid waste container
• PPE: Disposable gloves, eyewear

7210000 EnviroGard Triazine Test Kit by Modern Water

Envirogard® is a rapid field enzyme immunoassay method for the analysis of water. It uses a coated tube technology to detect the analyte of concern.

Features:

• The first environmental immunoassay format to be made commercially available
• Broad screen for the triazine family of herbicides including atrazine
• Rapid field method for the analysis of drinking and environmental water samples
• Test up to six tubes at once
• Coated tube immunoassay

Test result type

• Qualitative and Semi-quantitative data

Samples per kit

• 20 tube test kit (tests up to 12 samples)

Assay range

• 0.1 ppb to 1.0 ppb
• LDD = 0.053 ppb (atrazine)

Sample methods

• Water and a variety of other matrices

Sampling time

• Sample-to-data in approximately 10 minutes

Specificity

The EnviroGard® Triazine Test Kit does not differentiate between atrazine and closely related compounds, but detects their presence to differing degrees. The chart below shows the approximate Lower Limits of Detection (LDD) for the cross-reacting compounds.

Basic Test Procedure

• Add 160 uL of calibrator, negative control or sample to the tube
• Add four drops of enzyme conjugate
• Incubate for five minutes
• Decant and wash four times
• Add four drops of substrate and chromogen
• Incubate for two minutes
• Add one drop of stop solution
• Read on photometer

Test kit components

• 20 antibody-coated test tubes
• One vial of negative control
• One vial of atrazine-enzyme conjugate
• One vial of 0.1 ppb atrazine calibrator
• One vial of 1.0 ppb atrazine calibrator
• One vial each substrate and chromagen
• One vial stop solution
• One test tube rack

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use for up to 8 hours
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• Triazine EnviroGard® test kit (7210000)

Required test equipment

• Disposable-tip pipette which will measure 160 uL
• Timer
• Glass tubes or vials for calibrator preparation
• Tap or distilled water for rinsing tubes
• Marker pen
• Pipette that will measure 0.5mL

Other recommended materials

• Calculator
• Spectrophotometer

7310000 EnviroGard DDT Soil Kit by Modern Water

Envirogard® is a rapid field enzyme immunoassay method for the analysis of soil remediation, assessment and industrial testing. It uses a coated tube technology to detect the analyte of concern.

Features:

• Rapid and simple field testing procedures
• Semi-quantitative data results at set calibrator (action) levels
• Test up to 17 samples at once for increased cost efficiency
• Results available in less than 30 minutes
• Coated tube immunoassay
• EPA SW-846 Method #4042

Test result type

• Semi-quantitative or qualitative data

Samples per kit

• Up to 17 soil sample test results can be obtained per kit, depending upon the number of runs and action levels used.
• Typical applications use two or three calibrators and one negative control.

Assay range

• Semi-quantitative results (greater or less than) at calibrator (action) levels of 0.2, 1.0 and 10.0 ppm DDT
• Minimum detection level (MDL) is 0.2 ppm DDT.
• No maximum screening level as extracts can be diluted into test range

Sample preparation

• Soil samples require prior extraction using the MWI Sample Extraction Kit (sold separately).
• The Sample Extraction Kit provides material for 12 soil sample extractions with methanol.

Sampling time

• Soil extraction time is typically 2 minutes per sample plus assay run time of approximately 30 minutes.

Specificity

The EnviroGard DDT immunoassay test does not differentiate between DDT and other organochlorines. The table below shows compounds at the minimum detection limit (MDL) -an approximate concentration required to yield a positive result atthe lowest standard. The IC50 is the concentration required to inhibit one half of the color produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Basic Test Procedure

• Extract the soil sample using the MWI Sample Extraction Kit
• Pipet DDT assay diluent into the antibody coated tubes, then add either DDT calibrator or sample extract to the tubes along with DDT enzyme conjugate.
• Wait 15 minutes, then decant and wash the tubes.
• Add substrate.
• Wait for 10 minutes, add stop solution
• Read the absorbance using a photometer, and interpret the results. (Refer to DDT Soil Test Kit Instruction Guide procedures.)

Test kit components

• 20 antibody coated tubes
• Enzyme conjugate, colour developing and colour stop solution reagents
• Calibrators for 0.2, 1.0 and 10.0 ppm DDT.
• Negative control calibrator
• Test tube rack and pipet tips.
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Components must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight
• Portable spectrophotometer battery should be charged prior to use

Required test materials

• EnviroGard® DDT Soil Kit (7310000)
• Sample extraction kit (soil)(7310000EB)

Required test equipment

• Field soil lab (Accessory kit) (6050400) which includes: Repeating pipette*, Adjustable pipette*, Balance*, Repeating pipette tips*, Timer*
• Differential spectrophotometer - 110V (6000001), 220V (6000002)
• Field lab system rental (includes items marked with * above) (6997020)

Other recommended materials

• Latex gloves
• Absorbent paper for blotting test tubes
• Liquid and solid waste containers
• Calculator

Conf. with 3900000 (Extract), I00623B (Diluent 12/box) and 2 x 1005105 (Ampule Meth 20mL 6/box)

SD50951 QuickChek Sulphate Reducing Bacteria RC II Economy-Pak (100) by Modern Water

The QuickChek™ SRB Detection System is a rapid enzyme immunoassay method that detects sulfate reducing bacteria (SRB). The test employs purified antibodies to detect the enzyme adenosine-5'-phosphosulfonate (APS) reductase which is common to all strains of SRB.

The purified antibodies are attached to small particles that selectively capture the APS reductase enzyme. The particles and captured enzyme are then isolated on a porous membrane, which forms a reactive layer that turns blue in the presence of the APS reductase enzyme.

QuickChek™ SRB offers several advantages over cell culture techniques for SRB detection including immediate and accurate results. The kit allows testing of solid and semi-solid samples and detects all SRB, including SRB that is unable to grow in some standard media. The test results are not compromised by chemical or salinity interferences that are often found in field samples.

The QuickChek™ SRB kit is completely self-contained and disposable. Each kit includes all necessary materials to detect and enumerate sulfate reducing bacteria. There is no need for pretreatment or dilution of samples and there are not special hazardous waste disposal limitations.

Features:

• Semi-quantitative results
• Detects all viable and nonviable SRB samples
• Results available in approximately 8-10 minutes
• Two or three samples simultaneously
• Detection limit of 103 cells/ml
• etection limit for "clean" waters down to 101 cells/ml

Applications

• Corrosion coupons
• Cutting fluids
• Drilling fluids (Muds)
• Solids: biofilms, sludge, rust, mud
• Water associated with oil

A00071 Atrazine 100 Test Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil, food or water samples
• Quantitative data results with excellent analytical precision
• Results available in approximately 60 minutes
• Tests up to 50 samples at once
• Magnetic particle immunoassay
• Training available
• EPA SW-846 method #4670 for drinking water

Test result type

• Quantitative, semi-quantitative or qualitative depending on protocol selected

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• 0.04 to 5.0 ppb
• LDD = 0.1 ppb

Sample methods

• Methods exist for soil and a wide variety of commodities

Sampling time

• 'Sample-to-Data' in approximately 60 minutes

Specificity

Basic Test Procedure

• Add 200 μL of calibrator or prepared sample, 250μL enzyme conjugate and 500 μL antibody coupled magnetic particles.Vortex
• Incubate for 15 minutes
• Using the RaPID magnetic separator, decant and wash
• Add 500 μL colour solution
• Incubate for 20 minutes
• Stop the reaction and read colour at 450 nm
• Quantitive results and QC parameters are calculated and printed automatically using the RPA-II® spectrophotometer

Test kit components

• Antibody coated magnetic particles, enzyme conjugate, colour development, stop andwash reagents for analysis of 100 test tubes
• Standards for 0.1 1.0 and 5.0 ppb Atrazine
• Zero standard and 3.0 ppb control standard
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• Atrazine RaPID Assay (A00071)
• Atrazine sample diluent (100mL) (A00051)

Required test equipment

• Accessory kit (purchase) (6050100)
• Accessory kit (rental) (6997010)
• Accessory kit components: Magnetic separation rack (A00004), Repeator pipette (A00008), Adjustable volume pipette (A00176), Adjustable volume pipette (6000111), RPA-II analyser with printer (A00014), Vortex mixer (A00014), Digital timer (A00015)

7311000 EnviroGard Chlordane in Soil Kit by Modern Water

Envirogard® is a rapid field enzyme immunoassay method for the analysis of soil remediation, assessment and industrial testing. It uses a coated tube technology to detect the analyte of concern.

Features:

• Rapid field testing kit
• Semi-quantitative results at set calibrator (action) levels
• Test up to 16 samples at one time
• Results in less than 30 minutes
• Coated tube immunoassay
• Training available
• EPA SW-846 Method #4041

Test result type

• Semi-quantitative data or qualitative

Samples per kit

• Up to 16 soil samples depending on the number of runs and action levels used. (Kit includes 20 antibody coated tubes)

Assay range

• Semi-quantitative results (greater or less than) at calibrator (action) levels of 20, 100 and 600 ppb total Chlordane
• Minimum detection limit (MDL) is 20 ppb Chlordane
• No maximum screening level as extracts can be diluted into test range

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately)
• The sample extraction kit provides material for 12 soil sample extractions with methanol

Sampling time

• Approximately two minutes per sample plus assay run time of approximately 30 minutes

Sampling time

The EnviroGard® Chlordane immunoassay test does not differentiate between Chlordane and other cyclodienes. The table below shows compounds at the minimum detection limit (MDL) - an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross -reactivity values to similar compounds. The approximate Lower Limits of Detection (LDD) for the cross-reacting compounds.

Basic Test Procedure

• Extract the soil sample using the sample extraction kit
• Pipet Chlordane assay diluent into the antibody coated tubes, then add either calibrator or sample extract into the tubes
• Wait for 15 minutes
• Add Chlordane enzyme conjugate to the tubes
• Wait for five minutes, decant and wash the tubes
• Add substrate solution
• Wait for three minutes, add stop solution
• Read the absorbance using a photometer and interpret the results. (Refer to Chlordane Soil test kit instruction guide procedures)
• Stop the reaction and interpret results

Test kit components

• Assay diluent
• 20 antibody-coated test tubes
• Enzyme conjugate, substrate and stop solution reagents
• Calibrators for 20, 100 and 600 ppb Chlordane
• Negative control calibrator
• Test tube rack. and pipet tips
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight
• Portable spectrophotometer battery should be charged prior to use

Required test materials

• EnviroGard Chlordane in soil kit (7311000)
• Sample extraction kit (soil)(7311000EB)

Required test equipment

• Field soil lab (Accessory kit) (6050400) which includes: Repeating pipette*, Adjustable pipette*, Balance*, Repeating pipette tips*, Timer*
• Differential spectrophotometer - 110V (6000001), 220V (6000002)
• Field lab system rental (includes items marked with * above) (6997020)

Other recommended materials

• Latex gloves
• Absorbent paper for blotting test tubes
• Liquid and solid waste containers
• Calculator
• Tap or laboratory grade water for tube rinsing
• Marker pen

Warranty Plan includes annual calibration and cleaning, full parts and labour cover for failures (not those related to physical damage) plus shipping costs.