Modern Water, Test and Measurement

7311000 EnviroGard Chlordane in Soil Kit by Modern Water

Envirogard® is a rapid field enzyme immunoassay method for the analysis of soil remediation, assessment and industrial testing. It uses a coated tube technology to detect the analyte of concern.

Features:

• Rapid field testing kit
• Semi-quantitative results at set calibrator (action) levels
• Test up to 16 samples at one time
• Results in less than 30 minutes
• Coated tube immunoassay
• Training available
• EPA SW-846 Method #4041

Test result type

• Semi-quantitative data or qualitative

Samples per kit

• Up to 16 soil samples depending on the number of runs and action levels used. (Kit includes 20 antibody coated tubes)

Assay range

• Semi-quantitative results (greater or less than) at calibrator (action) levels of 20, 100 and 600 ppb total Chlordane
• Minimum detection limit (MDL) is 20 ppb Chlordane
• No maximum screening level as extracts can be diluted into test range

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately)
• The sample extraction kit provides material for 12 soil sample extractions with methanol

Sampling time

• Approximately two minutes per sample plus assay run time of approximately 30 minutes

Sampling time

The EnviroGard® Chlordane immunoassay test does not differentiate between Chlordane and other cyclodienes. The table below shows compounds at the minimum detection limit (MDL) - an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross -reactivity values to similar compounds. The approximate Lower Limits of Detection (LDD) for the cross-reacting compounds.

Basic Test Procedure

• Extract the soil sample using the sample extraction kit
• Pipet Chlordane assay diluent into the antibody coated tubes, then add either calibrator or sample extract into the tubes
• Wait for 15 minutes
• Add Chlordane enzyme conjugate to the tubes
• Wait for five minutes, decant and wash the tubes
• Add substrate solution
• Wait for three minutes, add stop solution
• Read the absorbance using a photometer and interpret the results. (Refer to Chlordane Soil test kit instruction guide procedures)
• Stop the reaction and interpret results

Test kit components

• Assay diluent
• 20 antibody-coated test tubes
• Enzyme conjugate, substrate and stop solution reagents
• Calibrators for 20, 100 and 600 ppb Chlordane
• Negative control calibrator
• Test tube rack. and pipet tips
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight
• Portable spectrophotometer battery should be charged prior to use

Required test materials

• EnviroGard Chlordane in soil kit (7311000)
• Sample extraction kit (soil)(7311000EB)

Required test equipment

• Field soil lab (Accessory kit) (6050400) which includes: Repeating pipette*, Adjustable pipette*, Balance*, Repeating pipette tips*, Timer*
• Differential spectrophotometer - 110V (6000001), 220V (6000002)
• Field lab system rental (includes items marked with * above) (6997020)

Other recommended materials

• Latex gloves
• Absorbent paper for blotting test tubes
• Liquid and solid waste containers
• Calculator
• Tap or laboratory grade water for tube rinsing
• Marker pen