Test and Measurement, Modern Water

A00205 RaPID Assay Carcinogenic PAHs (CPAH) 100 Tube Kit by Modern Water

RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.

Features:

• Rapid field testing procedure for analysis of soil and water samples
• Quantitative data results with excellent analytical precision
• Test up to 50 samples at one time
• Results available in approximately 60 minutes
• Training available
• Magnetic particle immunoassay
• EPA SW-846 method #4035 with increased sensitivity to carcinogenic PAHs

Test result type

• Quantitative, semi-quantitative or qualitative

Samples per kit

• 100 test kit (tests up to 80+ samples)

Assay range

• Soil: 10 ppb to 500 ppb as Benzo(a)pyrene
• Water: 0.1 to 5.0 ppb as Benzo(a)pyrene
• Range can be extended with additional dilutions
• Site specific calibration required to meet total PAH/C-PAH analysis requirements

Sample preparation

• Soil samples require prior extraction using the sample extraction kit (sold separately).
• The sample extraction kit provides materials for 12 soil sample extractions with methanol.
• Water samples must be diluted with an equal volume of methanol to prevent adsorptive loss.

Sampling time

• Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes

Specificity

The total Carcinogenic PAH concentrations (sum of seven Carcinogenic PAH compounds) of the indicated contaminant types in soil samples are expressed below, at each of the three kit calibrator levels, in units comparable to results from GC method 8015 (TPH).

The RaPID assay Carcinogenic PAH immunoassay test does not differentiate between benzo(a)pyrene and other polyaromatic hydrocarbons. The table below shows compounds at the limit of quantitation (LOQ)- an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.

Test kit components

• Antibody coated magnetic particles, enzyme conjugate, colour development and stop and wash solutions for analysis of 100 test tubes
• Standards for 0.1, 1.0 and 5.0 ppb as Benzo(a) pyrene
• Kit control as 2.0 ppb as Benzo(a)pyrene
• Disposable test tubes
• Test kit instructions

Storage & precautions

• Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
• Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
• Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
• Kits must be brought to 64° to 81°F (18° to 27°C) before use
• Do not expose colour solution to direct sunlight

Required test materials

• CPAH 100 tube kit (A00205)
• Sample extraction kit(for soil samples only) (A00204EA)
• cPAH sample diluent (100 mL) (A00203)

Required test equipment

• RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-II analyser (6000081), Printer (6000082), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
• Repeator pipet tips(12.5 mL) (A00009)
• Adjustable pipet tips (A00013)

Other recommended materials

• Latex gloves
• Liquid and solid waste containers
• Calculator
• Absorbant paper for blotting
• Marking pen

AZF50H100 MicrotoxOmni Software version 4.2 by Modern Water

Provided with purchase of Microtox® M500 analyser:

• ASTM (D5660)
• Basic toxicity test
• Comparison test
• Confirmation test
• ISO (International Standard Organization 11348-3)
• DIN (Deutsches Institut fur Normung 38412 Teil Test)
• Screening toxicity test
• Solid Phase/Basic Solid Phase
• Comparison
• Confirmation
• WET (Whole Effluent Toxicity)